Serum antibodies to HLA-E, HLA-F and HLA-G in patients with SLE during disease flares: Clinical relevance of HLA-F autoantibodies.

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Serum antibodies to HLA-E, HLA-F and HLA-G in patients with SLE during disease flares: Clinical relevance of HLA-F autoantibodies.

Clin Exp Immunol. 2015 Oct 6;

Authors: Jucaud V, Ravindranath MH, Terasaki PI, Morales-Buenrostro LE, Hiepe F, Rose T, Biesen R

Abstract
T-lymphocyte hyperactivity and progressive inflammation in SLE patients results in overexpression of HLA-Ib on the surface of lymphocytes. These are shed into the circulation upon inflammation, and may augment production of antibodies promoting pathogenicity of the disease. The objective was to evaluate the association of HLA-Ib (HLA-E, HLA-F and HLA-G) antibodies to the disease activity of SLE. The IgG/IgM reactivity to HLA-Ib and β2m in the sera of 69 German, 29 Mexican female SLE patients, and 17 German female controls was measured by multiplex Luminex®-based flow cytometry. The values were expressed as Mean Florescent Intensity (MFI). Only the German SLE cohort was analyzed in relation to the clinical disease activity. In the controls, anti-HLA-G IgG predominated over other HLA-Ib Abs, whereas SLE patients had a preponderance of anti-HLA-F IgG over the other HLA-Ib Abs. The disease activity index, SLEDAI-2000, was reflected only in the levels of anti-HLA-F IgG. Anti-HLA-F IgG with MFI level of 500 to 1999 was associated with active SLE; whereas inactive SLE revealed higher MFI (> 2000). When anti-HLA-F IgG were cross-reactive with other HLA-Ib alleles, their reactivity was reflected in the levels of anti-HLA-E and -G IgG. The prevalence of HLA-F-monospecific Abs in SLE patients was also associated with the clinical disease activity. Anti-HLA-F IgG is possibly involved in the clearance of HLA-F shed from lymphocytes and inflamed tissues to lessen the disease's severity, and thus emerges as a beneficial immune- biomarker. Therefore anti-HLA-Ib IgG should be considered as a biomarker in standard SLE diagnostics. This article is protected by copyright. All rights reserved.

PMID: 26440212 [PubMed - as supplied by publisher]